Separation of free radical scavenging compounds from Platanus orientalis leaves

Cancer causes mortality in millions of humans around the world, and due to its predominance, the discovery of anticancer compounds is interesting. Free radical scavenging (FRS) compounds are bioactive compounds that are widely present in nature and significantly delay or prevent cancers, chronic cardiovascular diseases, and aging by inhibiting free radicals. Considering the adverse effects of synthetic antioxidants on human health there is a growing interest to introduce new and potential sources of bioactive compounds. The current study studied the effect of various solvents including ethanol, methanol and n-hexane on bioactive compounds recovery from Platanus orientalis leaves. The extracts' anti-radical capacity (in terms of percentage inhibition of DPPH and HO free radicals) and total phenolic compounds were measured using spectroscopy methods. Moreover, the main phenolic compounds obtained were quanti/qualitatively measured using high-performance liquid chromatography. From the results, ethanolic extracts showed the highest anti-radical capacity (%DPPHsc of ۲۲.۱۱ ± ۰.۱۳ and %HOsc of ۱۶.۴۰ ± ۰.۱۴) and TPC (۲۲.۱۱ ± ۰.۱۸). Furthermore, catechin was the dominant compound determined in the extracts. Therefore, it could be stated that P. orientalis leaves are a potential source of valuable phytochemicals containing FRS compounds for further application in pharmacy, food, and cosmetic industries.

کلیدواژه ها:

Free radical ، Cancer ، Phenolic compounds ، Platanus orientalis leaves ، Chromatography.