Evaluating the effect of crocin on contrast-induced nephropathy in rats

Objective: Contrast-induced nephropathy (CIN) raises the risk of renal injury, but crocin, a saffron component, may improve kidney function. This study investigated crocin's protective effects against CIN in rats.Materials and Methods: Male Wistar rats were divided into eight groups: Sham, Control, Contrast medium (diatrizoate), Diatrizoate combined with crocin at ۱۰, ۲۰, or ۴۰ mg/kg/day, Diatrizoate combined with N-acetylcysteine (NAC) at ۱۲۵ mg/kg/day, and Crocin alone at ۴۰ mg/kg/day. Water deprivation began on day ۵ for ۴۸ hr, except for the sham and crocin alone groups. Indomethacin and N(ω)-nitro-L-arginine methyl ester were administered after ۴۰ hr of dehydration. Rats were sacrificed on the eighth day, and blood and kidney samples were collected.Results: Diatrizoate increased serum creatinine and blood urea nitrogen levels, elevated malondialdehyde levels, and reduced glutathione in renal tissue. Crocin reversed these effects. Diatrizoate caused severe tubular necrosis, proteinaceous casts, medullary congestion, and interstitial edema in kidney tissue. Crocin (۲۰ and ۴۰ mg/kg) significantly reduced tubular necrosis, and doses of ۱۰ and ۴۰ mg/kg reduced interstitial edema. NAC significantly improved histopathological damage, biochemical factors, and oxidative stress. The crocin alone group showed no significant changes.Conclusion: Diatrizoate induces nephrotoxicity by enhancing oxidative stress in rats, and crocin has a protective effect against it. Crocin mitigates both tissue and biochemical damage inflicted by diatrizoate.

کلیدواژه ها:

Antioxidants ، Diatrizoate ، Renotoxicity ، Indomethacin ، L- NAME ، Contrast media