Regulation of Spike Development through miRNAs in Barley (Hordeum vulgare L.)

سال انتشار: 1403
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 88

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شناسه ملی سند علمی:

ICSDA08_418

تاریخ نمایه سازی: 19 اسفند 1403

چکیده مقاله:

Barley (Hordeum vulgare) was one of the first cultivated cereal grains, giving it nonfragile spike making it much easier to harvest. It was domesticated in the Fertile Crescent including west of Iran. Understanding the genetic mechanism that regulates spike formation is important for studying domestication and breeding for yield improvement in barley. MicroRNAs (miRNAs) play a critical role in regulation of plant architecture including spike formation. However, miRNAs are less studied in barley. To identify miRNAs involved in spike development in barley, we performed whole transcriptome profiling of spike at early developmental stages using Illumina deep sequencing technology. A total of ۲۰ known miRNAs and ۸۵ putative novel miRNAs belonging to ۱۴ and ۷۹ miRNA families were identified, respectively. MiRNA target predictions showed that most of them are transcription factors (TF), including ۷ highly conserved TF families. The targets were involved in various biological functions including plant development, signal transduction, metabolic pathway, ion transportation, and environmental stresses in particular senescence genes. Our results provide new insights into the genetics of barley spike development that helps breeders to better understand the genetic determination of final yield in cereals and discovering the natural genetic resources for improving cereal yield through conventional or modern breeding approaches.

نویسندگان

Khadijeh Sattari

Master of Science graduate, Brindavan College, Bangalore University

Elahe Tavakol

Associate professor, Plant production and genetics, Shiraz University