The effect of glibenclamide on performance, carcass characteristics, blood parameters, immunity, intestinal microbial flora, intestinal morphology and breast muscle fatty acid profile in broilers

The effect of high levels of glibenclamide on performance, carcass characteristics, blood parameters, immunity, intestinal microbial flora, intestinal morphology and breast muscle fatty acid profile in broiler diets was investigated. ۱۲۰ One-day-old broiler chickens (n = ۱۲۰) of commercial strain Ross ۳۰۸ were randomly distributed into three treatments, each with ۴ replications including ۱۰ chickens in each pen. Experimental treatments included ۰, ۷۵ and ۱۰۰ mg/kg of glibenclamide. Data analysis was accomplished using SAS statistical software with comparison of averages at ۵% probability level using Duncan's multiple range test. Chickens were fed with diets based on corn and soybean meal. The results indicate that the use of different concentrations of glibenclamide in any of the rearing periods did not affect performance (P≥۰.۰۵), but the highest feed consumption, weight gain, and the best conversion rate were observed in periods ۱ to ۱۰, ۲۲ to ۴۲ and ۱ to ۴۲ days for ۷۵ mg/kg concentrations of glibenclamide. Also, lower production cost (P≥۰.۰۵) and the best European production index (P≥۰.۰۵) was observed when ۷۵ mg/kg glibenclamide was fed. Feeding glibenclamide did not affect chicken carcasses characteristics (P≥۰.۰۵) except that abdominal fat (P≥۰.۰۵) was reduced when ۷۵ mg/kg glibenclamide was fed. Feeding glibenclamide at ۷۵ mg/kg (P<۰.۰۵) reduced cholesterol, triglycerides, VLDL, LDL and total protein, whereas feeding ۱۰۰ mg/kg increased HDL. Except for SRBC۳۵, feeding glibenclamide had no effect on humoral immune system in response to antigen injection (SRBC antibody against sheep red blood cell). The highest relative weight of lymphatic organs was observed with ۱۰۰ mg/kg glibenclamide (P≥۰.۰۵). Glibenclamide fed at ۷۵ mg/kg reduced the number of Escherichia coli (P<۰.۰۵) and increased the number of lactobacillus acidophilus (P≥۰.۰۵). Villi length and crypt depth increased with ۷۵ mg/kg glibenclamide compared with the control. The ratio of saturated to unsaturated fatty acids was reduced with ۷۵ mg/kg of glibenclamide. The results of this trial indicate that feeding of glibenclamide has positive effects on performance, carcass characteristics, blood parameters, immunity, intestinal flora, intestinal morphology and fatty acid profile, this feed additive can be used as a substitute for antibiotics.

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