Nephroprotective impact of royal jelly against rhabdomyolysis-induced kidney damage in rats

Objective(s): The current work aimed at studying the impact of royal jelly on kidney damage caused by rhabdomyolysis in male rats.Materials and Methods: ۴۰ male rats were randomly assigned to five groups of eight: control, rhabdomyolysis, and rhabdomyolysis, administered with three doses of royal jelly (RJ) (۱۰۰, ۲۰۰, and ۴۰۰ mg/kg) for seven days. On the fifth day, we injected glycerol intramuscularly to induce rhabdomyolysis. Researchers examined serum biochemical parameters, inflammation, oxidative stress, apoptosis, and kidney tissue injury.Results: As a result of injecting glycerol, serum levels of creatinine, urea, and creatine phosphokinase were considerably elevated. The concentration of inflammatory mediators, as well as the expression of apoptotic parameters, was significantly elevated after glycerol injection. The percentage of kidney tissue damage and neutrophil gelatinase-associated lipocalin expression also increased significantly. Oral usage of RJ (۱۰۰, ۲۰۰, and ۴۰۰ mg/kg) caused a decline in serum CPK, tissue level of total thiols, catalase activity, and renal expression of   BAX compared to the rhabdomyolysis group. Serum creatinine and NGAL expression were also significantly reduced by the RJ (۲۰۰ and ۴۰۰ mg/kg). RJ significantly reduced the concentration of inflammatory mediators at ۱۰۰ and ۲۰۰ mg/kg doses and the expression of bcl۲ by RJ at ۱۰۰ and ۴۰۰ mg/kg doses.  Conclusion: Royal jelly safeguards the kidney from rhabdomyolysis-related damage, primarily through its anti-oxidant, anti-apoptotic, and anti-inflammatory effects.

کلیدواژه ها:

Acute kidney injury ، Apoptosis ، Inflammation ، Oxidative stress ، rhabdomyolysis ، Royal jelly