Assessment Of Anxiety -Like Behaviour And Antioxidant Levels In The Cerebral Cortex After Stroke Combined With Immobility Stress

Objectives: The assessment of anxiety-like behavior and antioxidant levels in the cerebral cortex after stroke combined with immobility stress explores the complex interplay between psychological stress and physiological responses in the context of stroke recovery. Methods: In this study, the rats were randomly assigned to four groups (n = ۱۰ each): sham, control (stroke induction), immobility stress (IS) (acute stress without stroke), and an immobility stress with stroke induction (ISS) group. To induce acute stress, rats were placed in transparent restrainers for ۱.۵ hours prior to surgery. Anxiety-like behaviors were evaluated using the Elevated Plus Maze (EPM), and total thiol concentration in the cortex was measured. Results: Statistical analysis confirmed that interventions significantly impacted EPM (p < ۰.۰۰۰۱), in the control, IS, and ISS groups compared to the sham group. Biochemical analysis of total thiol content in cortical tissue indicated a significant decrease in the control group compared to sham (p < ۰.۰۵) and in the ISS group relative to sham (p < ۰.۰۱). Notably, immobilization stress alone did not significantly affect thiol levels, as no differences were found between the IS and sham groups. Conclusion: Our exploration of the interplay between stress responses and oxidative stress highlights critical insights into the health of cortical tissue following stroke. The findings reveal that while stress can exacerbate anxiety-like behaviors, immobilization stress does not significantly compromise the antioxidant defenses in the cerebral cortex.

کلیدواژه ها:

Anxiety ، Antioxidant ، Stroke ، Stress