Phage Display Technology As a Tool For Vaccine Development

Vaccines are considered one of the most important bioproducts in medicine. Since the development of the smallpox vaccine in ۱۷۹۶, several types of vaccines for many diseases have been created. However, some vaccines have shown limitations as high cost and low immune responses. Bacteriophages (phages) are playing important roles in vaccine design, particularly in peptide selection and antibody development. Immunoglobulin G (IgG) from sheep which were protected from dermatophilosis with an enzyme preparation from Dermatophilus congolensis (D. congolensis), were used to select peptides displayed on phage in the Ph.D. -۷ random peptide library which contains ۱۰۹ peptides. Phage displaying peptides which were unrelated to the mimotopes associated with protection to dermatophilosis were removed with IgG from sheep which were vaccinated with the enzyme preparation but were not protected. The IgG from the protected sheep before vaccination was also used for negative selection. From the selected mimotopes those with clearly repeating motifs were chosen and used to vaccinate sheep. A mixture of four phage mimotopes induced antibody to a recombinant protease from D. congolensis. The vaccinated sheep, when challenged with two strains of D. congolensis, recovered more rapidly from the lesions caused by the strains.

کلیدواژه ها:

Vaccine ، Phage display ، Bacteriophage ، sheep