Introduction: Sugar beet seed production, unlike the cultivation of sugar beets which is a key component of sugar production in temperate regions worldwide, is a rare and lesser-known agricultural practice that occurs in specific climatic zones (Sadeghzadeh Hemayati et al., ۲۰۲۳). In Iran, the history of sugar beet seed production dates back approximately ۷۰ years to the establishment of the sugar beet production company. Initially, the Ardabil region was selected as the most suitable area for producing high-quality sugar beet seeds due to its climatic conditions (Alavi Samreen, ۱۹۹۸), and a commercial sugar beet seed production station was set up there. This study aimed to assess the difference between the potential or attainable yield of sugar beet seeds and the actual yield achieved by farmers in the Ardabil, and to identify the underlying causes. The research sought to quantify the yield gap in sugar beet seed production, determine the factors contributing to this gap, and assess the impact of each factor on the yield gap. Materials and methods: The study was conducted during the ۲۰۲۲-۲۰۲۳ growing season in Ardebil, Iran. Four prominent sugar beet cultivars - Shokoufa, Nika, Dena, and Arta - were selected for the investigation. These cultivars were propagated across ۸۷ farms, all of which were included in the study to ensure a comprehensive analysis. To gather the necessary data, a detailed questionnaire was utilized. This questionnaire was designed to capture a wide range of variables, totaling ۲۶۶, which encompassed various aspects such as soil characteristics, field management practices, climatic parameters, and the quantitative and qualitative attributes of the seeds produced by the farmers. This extensive data collection enabled a thorough examination of the factors influencing sugar beet seed production. The analysis of the yield gap and the contribution of each factor to this gap was performed using the Comparative Performance Analysis (CPA) method (Soltani et al., ۲۰۰۰). To determine the yield model, the relationship between all measured variables (quantitative and qualitative) and the packable seed yield was investigated through stepwise regression (Bie, ۲۰۰۰; Torabi et al., ۲۰۱۱). Results and Discussion: After conducting a step-by-step regression analysis, the production model for the packable seed yield variable was estimated in the form of Equation ۱. This model allowed for the estimation of the constituents of the packable seed yield gap and the contribution of each yield-limiting factor. The average and maximum yields of packable seeds for the studied farmers were determined to be ۴۵۷.۵۷ and ۸۶۹.۶۸ units per hectare, respectively. The yield model, as depicted in Figure ۱, estimated the average (actual) and maximum (attainable) yields of packable seeds as ۴۵۷.۵۷ and ۸۰۶.۲۹ units per hectare, respectively. The total estimated yield gap was equal to ۳۴۸.۷۳ units per hectare, representing ۴۳.۳% of the potential yield. This indicates that there is a gap of ۳۴۸.۷۳ units per hectare between the actual yield achieved by Ardabil farmers and the yield they could achieve with optimal management. Among the various agronomic variables affecting the packable seed yield, the factors with the greatest impact on creating the yield gap were identified. These included the distance between planting and the second irrigation (۳۲.۲۸%), the amount of nitrogen application in the second stage (۲۵.۵۱%), the amount of silt in the soil (۲۰.۱۴%), the propagated cultivar (۱۳.۷۷%), and soil leveling (۸.۳۱%). These findings highlight the significant influence of these variables on the yield gap and suggest areas where improvements in management practices could lead to increased productivity. Conclusion: The study identified a substantial yield gap in sugar beet seed production in Ardabil, Iran, with a significant difference between actual and attainable yields. Key factors contributing to this gap include the timing of irrigation, nitrogen application, soil silt content, cultivar selection, and soil leveling. Improving these aspects of farm management could significantly enhance productivity, reducing the yield gap and increasing the efficiency of sugar beet seed production in the region.