Evaluation of antifungal activity of essential oils of Thymus vulgaris, Petroselinum Crispum, Cuminum cyminum and Bunium persicum on candida albicans in comparison with Fluconazole

Objective: Nowadays notable increase in acquired resistance of Candida species to antifungal drugs and necessity of using agents with antifungal properties is unavoidable. In some plants due to presence of components such as polyphenols have antimicrobial properties. In this study antifungal effects of essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum on standard strain of Candida albicans were evaluated. Materials and Methods: ۲۵ grams leafs of the Thymus vulgaris and Petroselinum crispum and seeds of the Cuminum cyminum and Bunium persicum were dried and grinded after that the essential oils of each mentioned plant were prepared by Clevenger system. Serial dilutions of essential oils were made in ۹۶ well microtiter plates. Minimum Inhibitory Concentration (MIC) and Inhibitory zone diameter were assessed by Microdilution broth and Disc diffusion agar techniques, MIC۵۰, MIC۹۰ and MFC were also determined. Results: Minimum inhibitory concentration ۹۰(MIC۹۰) essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum were respectively ۲۵, ۷۲, ۴۱۲, ۱۳۰ µg/ml and Minimal Fungicide Concentration (MFC) were ۴۸, ۱۴۶, ۶۲, ۲۸۰. Inhibitory zone diameters were ۲۸, ۲۰, ۱۲, ۱۵ millimetres. Conclusion: In this evaluation essential oils of Thymus vulgaris, Petroselinum crispum, Cuminum cyminum and Bunium persicum showed suitable antifungal effects against growth of standard strain of Candida albicans. Thus these herbal essences after supplementary studies possibly can be suitable substitute for chemical medicine on Candida infections especially on mucocutaneous Candidiasis.

کلیدواژه ها:

Antifungal effects ، Candida albicans ، herbal essences ، Fluconazole ، کاندیدا آلبیکنس ، آثار ضد قارچی ، فلوکونازول ، اسانس های گیاهی