Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients

Objective: In this study quantitative expression of MDR۱ and hOCT۱ genes in CML patients and normal people were measured using Real-Time PCR. Materials and Methods: To study quantitative expression of these genes by real-time PCR, master-mix with syber green was used. Peripheral blood samples from ۳۰ CML patients and ۲۷ normal persons were harvested. Real-time PCR results were analyzed with relative quantification method. Result: This study showed that in the patients group who were under treatment with Imatib, MDR۱ gene expression was increased which was statistically significant. This increase has a direct relation with disease progress. Gene expression in AP and BP patients was also higher than CP patients. In contrast, hOCT۱ expression in patients group in comparison with normal group was not statistically significant. Conclusion: MDR۱ increase in leukemic cell membrane results in the reduction of intra-cellular drug concentration. Thus, optimal concentration of drug for inhibition of BCR-ABL tyrosine kinase is not achieved which culminated in disease progression to AP and BP phases. Moreover changes in hOCT۱ gene expression as an influx transporter of Imatib could affect intracellular concentration of drug and finally determine therapy outcome. However, in this study hOCT۱ gene expression was variable and was not statistically significant.

کلیدواژه ها:

Real-Time PCR ، MDR۱ ، hOCT۱ ، Real-Time PCR ، MDR۱ ، hOCT۱ ، CML ، Gene expression ، لوسمی میلوئیدی مزمن ، بیان ژن