Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells

Objective: Cell vital function has correlation with mechanical loadings that cell experiences. Here, effects of in-vitro combined cyclic-static stretch on proliferation of human mesenchymal stem cell (HMSC) were evaluated. Materials and Methods: HMSCs were cultured on gelatin coated elastic membranes, and exposed to stretch loading. Four different regimes of cyclic, static, combined cyclic-static, and cyclic with a period of unloading were exerted on the elastic membrane. Duration of cyclic loading and static loading was ۵ and ۱۲ hours respectively. Results: The results illustrate that ۱۰% cyclic stretch causes cell alignment but there were no significant proliferation differences between control and test group. Combined cyclic-static stretch reduced proliferation significantly while cyclic stretch with an unloading period increased cell proliferation significantly. At last, static stretch did not affect cell proliferation significantly. Conclusion: Cell stretching regimes and post-loading duration are effective factors on cell proliferation.

کلیدواژه ها:

Tissue engineering ، Static Stretch ، Cyclic Stretch ، Cell Proliferation ، Human Mesenchymal Stem Cell ، مهندسی بافت ، کشش ثابت ، کشش دورهای ، تکثیر سلول ها ، سلول بنیادی مزانشیمال انسانی