The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast

Objective: Average Age of population in the industrial countries has increased. Because of aging the percent of the diseases related to the oldness such as multiple myeloma have also increased. It has both common and unique symptoms and effects. The unique effects include wide bone reabsorption. It seems necessary to understand the structure of Bone Marrow Niche and the effects of Myeloma cells on adjacent hematopoietic Stem cells with a new approach. Materials and Methods: We have studied the differentiating effect induced by the Myeloma cells through co-culturing the Myeloma cells and hematopoietic stem cells, extracted out of cord blood. In this investigation we also cocultured myeloma cells with the monoblastic cell line (U۹۳۷) in order to evaluate the effect of myeloma cells on monoblastic cells differentiation. Results: Our findings show that increased expression of myeloid and monocytoid markers in coculturing of myeloma cells and HSCs. Moreover following monoblastic and myeloid cells coculturing, we observed probably TRAP positive osteoclastic like cells. Conclusion: Our findings show that presence of myeloma cells in Bone Marrow play essential role in HSCs differentiation to monocytoid (osteoclastic) lineage.

کلیدواژه ها:

Niche ، Multiple Myeloma ، Hematopoietic Stem Cells ، Osteoclast Differentiation ، کنام ، مالتیپل مایلوما ، سلول بنیادی خونساز ، تمایز استئوکلاست