Antibacterial effect of the Persian Gulf sea cucumber Holoturia. SP extracts on three strain of Escherichia coli

Objective: Sea cucumber is a traditional food and medical item and has been reported to exhibit Antioxidant, antifungal, anti tumoral and antibacterial bioactivity. The objective of this study is to describe the antibacterial activity of ۴ extracts of Holoturia. Sp (sea cucumber), collected from Hengam Island of Persian Gulf. Materials and Methods: Methanol, hexane, aqueous and chloroform extracts from body wall tissue of the sea cucumber were screened for antibacterial activity against three strains of Escherichia coli Top ۱۰ F´, TG۱ and K۱۲ using disc diffusion and broth microdilution tests methods. Result: The growth of all these strains were inhibited using concentration from ۰.۷۸ to ۱۰۰ mg/ml of methanol, hexan and chloroform extracts. Among the extracts just methanol and chloroform with ۱۰۰ mg/ml had bactericidal effect on TG۱ and K۱۲ strains. On the other hand, Aqueous extract had induced growth in of the all strains. Conclusion: The results suggest the possibility of applying sea cucumber as source of potential anti bacterial agents, whose compounds can be good candidates to make antibiotic products.

کلیدواژه ها:

Antibacterial ، Sea cucumber ، Disc diffusion ، Broth microdilution tests ، ضد باکتریایی ، خیار دریایی ، انتشار دیسک ، ریزرقیق سازی محیط کشت