The effect of blood storage on apoptosis induction of Jurkat cells: an in vitro model

Objective: It is a firm belief that blood transfusion is life-saving in many situations, but at the same time transfusion complications could be life-threatening. The possible effects of blood Transfusion Related Immunomodulatory (TRIM) and its related mechanisms is one of the important debatable subjects in the field of blood transfusion medicine. One of the mechanisms through which transfusion can induced TRIM effects in recipient is apoptosis induction. Aim of this study was to investigate the apoptotic effects of stored blood in an in vitro model. Materials and Methods: To evaluate the apoptotic effects of blood storage, we studied the effect of the plasma (from whole blood) during storage on days ۳, ۱۰, ۲۱ and ۳۵ on Jurkat cells, which are sensitive to apoptosis .The plasma of whole blood was separated by centrifugation on different days. Then, Jurkat cells were cultured with plasma for ۲۴ hours. Finally apoptosis level was studied by using flowcytometry for analyzing Annexin V on Jurkat cells (by SeroTec Annexin V:FITC Assay Kit). Results: The percentages of apoptosis on days ۳, ۱۰, ۲۱ and ۳۵ were ۳.۸۵±۱.۵۲, ۵.۲۷±۲.۱۲, ۸.۴۴±۱.۹۰, ۱۲.۰۱±۲.۳۲, respectively. The percentages of apoptotic cells in negative and positive control group was ۳.۸۵±۱.۹۴ and ۶۵.۸۰±۲.۲۸, respectively. Conclusion: The results of this study showed that plasma of whole blood have the apoptotic effects which enhanced during the storage of plasma. This in vitro model is also suitable for studying other TRIM related mechanisms.

کلیدواژه ها:

Apoptosis ، TRIM ، Annexin V ، Jurkat cell ، مرگ برنامه ریزی شده سلولی ، تعدیل ایمنی ناشی از انتقال خون ، انکسین V ، سلول جرکات