Effect of In Vitro Gastrointestinal Digestion on Monacolin K and α-Glucosidase and α-Amylase Inhibitory Activities of BRTA and Chemometrics Analysis

Introduction: Broken rice tea supplemented with angkak (BRTA) contains monacolin K and Maillard reaction products (MRPs). The dynamic changes in these bioactive compounds and activities of BRTA after in vitro gastrointestinal digestion were investigated. Materials and Methods: Different ratios of roasted broken rice to angkak were supplemented in BRTA as ۲۵:۷۵, ۵۰:۵۰, ۷۵:۲۵, and ۱۰۰:۰. The colors, water activity (aw), moisture content, monacolin K, inhibition of peroxidation, DPPH radical scavenging assay, chelating ability on Fe۲+, and α-glucosidase and α-amylase inhibitory properties of BRTA were investigated after in vitro gastrointestinal digestion. Results: Monacolin K, antioxidant properties, and α-glucosidase and α-amylase inhibitory abilities of BRTA for all ratios of roasted broken rice to angkak increased after in vitro gastric digestion. BRTA activities using a ratio of ۲۵:۷۵ (roasted broken rice:angkak) were the highest. After in vitro gastrointestinal digestion, monacolin K, antioxidant properties, and α-glucosidase and α-amylase inhibitory abilities of BRTA decreased due to the alkaline condition. Principal component analysis (PCA) and Pearson’s correlation coefficient showed that monacolin K was positively correlated with α-amylase inhibition activity. Conclusions: Changes in monacolin K and α-glucosidase and α-amylase inhibitory abilities after in vitro gastrointestinal digestion of BRTA were investigated. Other experiments using angkak as a main ingredient should be conducted to determine dynamic changes in bioaccessibility.

کلیدواژه ها:

Angkak ، antioxidant properties ، PCA ، Pearson’s Correlation ، DPPH