Puerarin alleviates renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via Nrf۲/HO-۱ pathway

Objective(s): To explore the effects of puerarin on renal ischemia/reperfusion injury and the possible mechanism.Materials and Methods: The experimental mice were injected with puerarin (۵۰ or ۱۰۰ mg/kg) per day or equal sterile saline by intraperitoneal injection for one week, and a renal I/R injury model was constructed. HK-۲ cells were incubated with puerarin (۱ uM and ۱۰ uM) before the H/R model. Immunohistochemistry, immunocytochemistry, and Western blot analysis were used to detect the protein associated with apoptosis and endoplasmic reticulum stress.Results: Puerarin could improve renal function and attenuate tissue structural damage after renal I/R. Meanwhile, puerarin alleviated apoptosis and endoplasmic reticulum stress by decreasing expression levels of specific biomarkers such as caspase-۳, GRP۷۸, CHOP, and p-elF۲α/ elF۲α in animals and HK-۲ cells. The up-regulated expression of Nrf۲ and HO-۱ protein after puerarin treatment indicated that the Nrf۲/HO-۱ signaling pathway might mediate the protective mechanism of puerarin against renal I/R.Conclusion: Our results suggest that puerarin alleviated renal ischemia/reperfusion injury by inhibiting apoptosis and endoplasmic reticulum stress via the Nrf۲/HO-۱ pathway and offered new insights for preventing and treating renal I/R.

کلیدواژه ها:

Apoptosis ، Endoplasmic reticulum- stress ، Nrf۲/HO-۱ pathway ، Puerarin ، Renal ischemia/reperfusion- injury