In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia

Diabetes mellitus is one of the most widely occurring non communicable diseases spreading rapidly worldwide that occurs as a result of non-regulation of glucose in the blood stream or because of improper functioning of the enzymes α- amylase and α-glucosidase. Endophytes are the microorganisms that reside symbiotically inside the living tissues of plants. Endophytic fungi have the ability to synthesize various important bioactive metabolites. The current study was aimed to explore the less reported endophytic fungus Aspergillus austwickii isolated from ethnobotanical medicinal plant Premna serratifolia L. The methanolic extract of the endophytic fungus was subjected to activities like in vitro antioxidant assays, anti-inflammatory assay, antidiabetic assay in addition to exploring their total phenolic and total flavonoid content along with the phytochemicals present. The results revealed that Aspergillus austwickii exhibited phytoconstituents like alkaloids, phenols, flavonoids, tannins and carbohydrates. The total phenolic content and total flavonoid content of the fungus was found to be ۲۲.۰۴۸ µg GAE/g and ۱۸.۸۲۸ µg GAE/g. The crude extract showed ۴۶.۲۰+۰.۵۳% of antioxidant activity with an IC۵۰ value of ۱۲۸.۶۹ µg/mL for radical scavenging by DPPH. It also exhibited ۷۱.۸۶+۰.۳۴% of anti-inflammatory activity by protein denaturation assay. Notably, it revealed, antidiabetic activity for both alpha amylase and alpha glucosidase with the percentage of inhibition of ۶۸.۲۲+۰.۱۷% and ۷۳.۷۲+۰.۱۸% with an IC۵۰ value of ۱۷۸.۱۰ µg/mL and ۱۶۶.۱۶µg/mL respectively. The current study shows that the methanolic extract of Aspergillus austwickii possesses considerable antioxidant, anti-inflammatory and antidiabetic capabilities. The findings of the current research can be explored for the future research to find out new set of natural drugs which can be used against several disorders.

کلیدواژه ها:

Antioxidant ، alpha(α) amylase ، alpha(α) glucosidase ، Anti-inflammatory activity