The effect of Plasmodium berghei on intestinal cancer suppression in BALB/c mice

: Colon cancer is one of the most common diseases of the digestive system. Plasmodium, one of the most important parasites, can be considered for the treatment of cancer, and good studies have been done in this regard in the past. Our goal in this study is to investigate the effect of Plasmodium berghei in suppressing intestinal cancer in mice. Method: CT۲۶.WT mouse colon cancer cell line was purchased and used in (RPMI) ۱۶۴۰ medium (Gibco, USA) containing ۱۰% embryos Bovine serum (FBS) and penicillin-streptomycin ۱% (۱۰۰ units/ml of penicillin and ۱۰۰ μg/ml of streptomycin) under ۳۷°C and ۵% CO۲ in an incubator. Ten BALB/c mice were inoculated subcutaneously with ۰.۱ ml of CT۲۶.WT cell suspension. (۱۰۶ x ۵/ml) for each mouse's right forelimb axilla. On the day of tumor formation (on the sixth day After tumor cell injection), ۱۰ mice were randomly selected They were divided into two groups (۵ animals in each group). in CT۲۶.WT+P.y group, as Plasmodium berghei infected group, ۱x۱۰۶ was injected intraperitoneally into each mouse with Red blood cells infected with Plasmodium berghei. Control group mice (CT۲۶.WT) were injected intraperitoneally. Apoptosis was assessed by dUTP deoxynucleotidyl transferase (TdT) staining and the expression of apoptosis-related proteins including Bax, Bcl-۲, caspase-۹, and cleaved caspase-۳ by western blotting. Blot and immunohistochemistry respectively. A transmission electron microscope (TEM) was used to observe the ultrastructural changes in Colon cancer cells and the expression of central protein related to mitochondrial biogenesis, PGC-۱α, and mitophagy. The relevant important proteins, PINK۱/Parkin, were detected by western blotting. Result: We found that Plasmodium infection decreased the weight and size of tumors and decreased the expression of Ki۶۷ in colon cancer-bearing mice. Furthermore, Plasmodium infection promotes mitochondrial-mediated apoptosis in colon cancer cells, as up-regulated by increasing the proportion of TUNEL-positive cells. Bax, caspase-۹, and cleaved caspase-۳ proteins and low expression of Bcl-۲ protein. In colon cancer cells, we found degraded cell nuclei, swollen mitochondria, missing cristae, and a reduced number of autolyzed outcomes. In addition, Plasmodium infection impaired mitochondrial biogenesis and mitophagy. Expression of PGC-۱α, PINK۱, and Parkin proteins in colon cancer cells. Conclusion: Because of the high incidence and mortality associated with colon cancer, researchers to reduce Mortality and improve the quality of life of patients are continuously looking for new treatments to inhibit the growth of colon cancer, so we investigated this issue in this study.

کلیدواژه ها:

Plasmodium berghei ، intestinal cancer ، suppression ، BALB/c mice