Investigating the effect of coumarin on the expression of selected cellular lncRNAs (lncRNA-H۱۹, RNCR۳, and LINC۰۰۶۵۷) and their target genes (miR-۴۹۱-۵p, miR-۱۹۰a-۳p, PTEN, and miR-۱۸۵-۵p) in gliobla

: Glioma represents a complex and heterogeneous disease, posing significant challenges to both clinicians and researchers. Despite notable advancements in glioma treatment, the overall survival rate for most glioma patients remains dishearteningly low. Dietary natural substances are therefore evaluated for their potential as agents in GBM treatment. Various substances found in fruits, vegetables, and other natural products restrict tumor growth and induce GBM cell death. These preclinical effects are promising but remain constrained by natural substances’ varying pharmacological properties. Coumarins as a natural compound can act as anti-tumor agents by inhibiting DNA-associated enzymes like topoisomerase, causing cell apoptosis, modifying estrogen receptors, or blocking the cell cycle. Because long non-coding RNAs (lncRNAs) can affect several interconnected processes, its value as a predictive marker for glioblastoma has been demonstrated. Coumarin - a natural compound known to contain some beneficial antitumor qualities - was tested for its effects on U۸۷ glioblastoma cells. Method: In this study, we investigated the expression level of selected cellular lncRNAs H۱۹, RNCR۳, and LINC۰۰۶۵۷ and their target genes miR-۴۹۱-۵p, miR-۱۹۰a-۳p, PTEN, and miR-۱۸۵-۵p in coumarin-treated U۸۷ glioblastoma cell line. The expressions of the three lncRNAs: BANCR, MALAT۱ and FER۱L۴, as well as their specified targets, PTEN, PI۳K and AKT, were measured by qRT-PCR. To gauge the impact of coumarin on the glioblastoma cells, a MTT assay was utilized. Results: The experiment's results showed that glioblastoma viability diminished with increasing doses of coumarin. The findings demonstrated that the use of coumarin in U۸۷ cells resulted in reduced levels of RNCR۳ , H۱۹, miR-۱۸۵-۵p, miR-۱۹۰a-۳p, and miR-۱۸۵-۵p. Conversely, levels of LINC۰۰۶۵۷, PTEN and miR-۱۸۱a-۵p were significantly increased in cells treated with coumarin compared to those left untreated. Conclusion: This information points to coumarin being a possible option in a treatment regimen for glioblastoma due to its ability to affect lncRNAs and the molecules they target.

کلیدواژه ها:

Glioblastoma ، Long non-coding RNA ، H۱۹ ، RNCR۳ ، LINC۰۰۶۵۷