Biosynthesis of Silver Nanoparticles Using Oscillatoria Extract and Evaluation the Anticancer and Antibacterial Activities

The emergence of nanotechnology is one of the most promising areas for medical research. Today, biological methods of synthesizing nanoparticles have been considered in the fight against many diseases. Since the genus Osillatoria has more biologically active compounds, it seems that it has the regenerating ability of silver salt. Therefore, the use of their extract for the biosynthesis of metal nanoparticles is of great importance. Therefore, in the present study, the biosynthesis of bread and silver particles was carried out with the help of precipitation method with the regeneration of silver ions by the extract of cyanobacteria.The purpose of this study was to evaluate the anti-cancer and anti-bacterial activity of silver nanoparticles, biosynthesized with cyanobacteria acetate extract. Methods: In the present experimental study, the silver nanoparticles biosynthesis was performed using silver ions regeneration with cyanobacteria acetate extracts. Techniques such as X-ray diffraction, scanning electron microscopy and transient evaluation of silver nanoparticles were evaluated. In order to investigate the antibacterial activity of synthesized nanosilver, serial dilution method was used for broth microdilution test to determine minimum inhibitory concentration (MIC). The effects of silver nanoparticle toxicity on T۴۷D breast cancer cell line were evaluated using MTT colorimetric method. Also, the proximal anxine ۰.۵ propidoid yodide kit and flow cytometry system were evaluated to evaluate the percentage of apoptosis and necrosis in cancer cells treated with silver nanoparticles. Results: Characterization of biosynthetic silver nanoparticles indicated that these nanoparticles had a mean size of ۳۰ nm with dominant spherical morphology. The evaluation of the antibacterial properties of biosynthetic nanoparticles showed that the minimum inhibitory concentration for Escherichia coli, Acinetobacter Bumanni and Staphylococcus aureus was ۲۵, ۵۰ and ۱۲.۵ μg / ml, respectively. The results of cell proliferation of nanoparticles showed that its effect depends on the concentration and time of treatment of silver nanoparticles on cancerous cells. In addition, flow cytometric results showed an apoptotic cell death rate of ۳۵% in the T۴۷D cell line. Conclusion: Biosynthesis nanoparticles have anticancer and antibacterial activity and can be studied further in the treatment of breast cancer and infections caused by pathogenic bacteria.Since the genus Osillatoria has more biologically active compounds, it seems that it has the regenerating ability of silver salt. Therefore, the use of their extract for the biosynthesis of metal nanoparticles is of great importance. Therefore, in the present study, the biosynthesis of bread and silver particles was carried out with the help of precipitation method with the regeneration of silver ions by the extract of cyanobacteria.

کلیدواژه ها:

Anticancer ، Apoptosis ، Osituria ، Antibacterial