Abstract
Colorectal cancer (CRC) is a significant health problem as the third most common
cancer in the world and the second deadliest
cancer in the world. The aim of the present study was to evaluate expression of two genes (DDX۲۱ and mir۲۰۰a) in Iranian
CRC patients tissue samples. In this study, ۶۰ patients with colorectal
cancer and ۶۰ patients with no evidence of
CRC were enrolled. RNA extraction from The paraffin blocks of colorectal tissue samples and polyps was performed and expression level of DDX۲۱ and mir۲۰۰a genes was assessed using qPCR method. The results showed that mean age of patients was ۵۸.۳ ± ۹.۸۲ years and ۵۳.۷ ± ۸.۶۵ years in the case and control groups respectively. The expression of DDX۲۱ was significantly increased in
CRC patients (with a mean fold-change of ۱.۸, P<۰.۰۰۱). Additionally, the expression of miR-۲۰۰a was significantly increased in
CRC patients (with a mean fold-change of ۲.۵, P<۰.۰۰۱). In conclusion, DDX۲۱ and mir۲۰۰a seem to be valuable markers in prognosis of CRC..... Methods Study Design and Patients In this study, ۶۰ patients with colorectal
cancer and ۶۰ controls (with no evidence of CRC) were enrolled. The paraffin blocks of colon and rectum tissue samples in the case group and polyps in the control group, were prepared by the Pathology Department of Imam Hossein hospital (Tehran, Iran). The results of histopathological studies confirmed
CRC diagnosis in the case group patients. RNA isolation and qPCR Isolation of RNA performed in using the MiRJia Kit (Rojeh Technology, Iran) and were used for cDNA synthesis with random hexamers and Superscript III Reverse Transcriptase according to manufacturer’s instructions (ThermoFischer). cDNA was used for quantitative PCR analysis in a Step one real-time PCR cycler (Applied Biosystem), using SYBR Green Master Mix and gene-specific primers. The DDX۲۱ gene primer sequences were (Forward): CGGAGGACGGGGTGAAGAT and (Reverse): AAGAAGGTTCTGAACAGCTGGTTAG. Primer sequences of miR-۲۰۰a were (Forward): UAACACUGUCUGGUAACGAUGU and (Reverse): UAACACUGUCUGGUAACGAUGU.