The Effect of Adjuvant Therapy on Expression of Immune Checkpoints in Ovarian Cancer Patients

Background and Aims: Ovarian cancer (OC) occurs when aberrant cells proliferate and form a tumor, which is known as ovarian cancer, mostly of unknown causes. It is crucial to investigate the effect of biotherapies like Bevacizumab (Avastin) and chemotherapy on the gene expression of PD۱ and PDL۱ in ovarian cancer patients to understand the molecular dynamics of therapeutic responses. The present study was conducted with aim to investigate the effect of adjuvant therapy on the expression of a gene on the exterior of T-cells “Programmed cell death ۱” (PD۱) and on tumor cells “Programmed cell death ligand ۱” (PDL۱) in OC patients.Methods: The study included ۲۴ OC patients who were divided into three groups: Group ۱ (G۱) with ۱-۶ doses of Avastin, Group ۲ (G۲) with >۶ doses of Avastin, and Group ۳ (G۳) receiving chemotherapy, along with ۸ healthy controls. Gene expression of PD۱ and PDL۱ was analyzed using Real-Time PCR.Results: PD۱ and PDL۱ did not show significant differences between the study groups (P=۰.۶۲ and P=۰.۱۵۳, respectively). Additionally, there was a strong correlation between PD۱ and PDL۱ expression in all groups (P=۰.۰۰۱۲, P=۰.۰۰۶, and P=۰.۰۱۰ for G۱, G۲, and G۳, respectively). The gene PD۱ was downregulated in patients compared to controls (P=۰.۶۳۷, P=۰.۷۳۰, P=۰.۶۹۷, and P=۱.۰۰۴ for G۱, G۲, G۳, and control, respectively). Similarly, the gene PDL۱ was downregulated in patients (P=۰.۳۴۰, P=۰.۲۰۹, P=۰.۴۷۷, and P=۱.۰۰۰ for G۱, G۲, G۳, and control, respectively).Conclusion: The expression of PD۱ and PDL۱ and their correlation in ovarian cancer patients may indeed have a significant impact on evaluating therapeutic responses in these patients. Understanding the gene expression profiles of PD۱ and PDL۱ could provide valuable insights into the immune response and potential treatment outcomes for ovarian cancer.

کلیدواژه ها:

Ovarian Cancer ، Programmed Cell Death ، Programmed Cell Death Ligand ، Bevacizumab ، chemotherapy ، biotherapy