Potential of new fungicide production from phthalides of Kelussia odoratissima

A bioassay-guided fractionation on repeated silica gel column chromatography was used to identify natural antifungal agents in the ethanol extract of Kelussia odoratissima leaves. The ethyl acetate fraction of the extract has significant inhibitory activity against Macrophomina phaseolina (Tassi) Goid, whereas the water fraction showed no effect. The main compound responsible for antifungal activity was determined by NMR and Mass Spectrometry techniques and identified as (Z)-۳-butylidenephthalide. It exhibited the highest mycelial growth inhibition with IC۵۰ = ۲۷۰ mg/L. Additionally, it inhibited the microsclerotia production and dark pigments formation. (Z)-ligustilide was identified as a moderately active compound with ۶۲% inhibition. These findings revealed the crucial role of natural phthalides as sources of bioactive compounds that might be used in natural pesticide discovery.

کلیدواژه ها:

Macrophomina phaseolina ، (Z)-۳-butylidenephthalide ، (Z)-ligustilide ، natural products ، Lead compound