The protective properties of resveratrol against glycerol-induced acute kidney injury in rats

Objective: After rhabdomyolysis, muscle tissue releases substances such as myoglobin, creatine kinase, and electrolytes into the bloodstream, potentially leading to acute kidney injury (AKI). Resveratrol (RSV) is a polyphenol compound with anti-inflammatory and antioxidant effects and it is found in various plants. This research evaluated the protective effects of RSV in rhabdomyolysis-induced AKI in rat kidneys.Materials and Methods: Thirty-six male Wistar rats were randomly assigned to six groups (n=۶): ۱) control (normal saline), ۲) glycerol only (۱۰ ml/kg, intramuscular), ۳, ۴, and ۵) glycerol +RSV (۵, ۱۰, and ۲۵ mg/kg, intraperitoneal injection) and ۶) RSV (۲۵ mg/kg). After ۴ days, pathological alterations and the level of blood urea nitrogen (BUN) and serum creatinine were determined. Malondialdehyde (MDA), glutathione (GSH), neutrophil gelatinase-associated lipocalin (NGAL), and tumor necrosis factor-α (TNF-α) proteins were investigated in rat kidneys. Results: Injection of ۵۰% glycerol (۱۰ ml/kg, IM) resulted in pathological lesions, elevated levels of MDA (p<۰.۰۰۱), BUN (p<۰.۰۱), serum creatinine (p<۰.۰۰۱), TNF-α (p<۰.۰۱), and NGAL protein (p<۰.۰۰۱), and decreased GSH content (p<۰.۰۰۱) compared to the control animals. These findings indicated AKI induced by rhabdomyolysis. RSV (۲۵ mg/kg) administration significantly decreased serum creatinine, BUN, MDA, NGAL, and TNF-α levels compared to the glycerol group. Histopathologically, tubule necrosis, myoglobin cast formation and glomerular atrophy increased in the glycerol group and reduced in animals that received RSV.Conclusion: In the glycerol-induced AKI rat model, RSV administration alleviated renal dysfunction by reducing oxidative stress and inflammatory responses.

کلیدواژه ها:

rhabdomyolysis ، Acute renal failure ، Grape ، Inflammation