Exploring the Role of the USP Superfamily in Teratozoospermia: AComprehensive Meta-Analysis

Introduction: Teratozoospermia is a significant factor contributing to male infertility and ischaracterized by abnormal sperm morphology. Deubiquitinating enzymes play vital roles in thecontrol of protein synthesis and degradation during spermiogenesis. Methods: The transcriptomedata of teratozoospermia and normozoospermia samples from the GEO database was analyzed.Three eligible GSE profiles of teratozoospermia (GSE۶۹۶۷, GSE۶۹۶۸ and GSE۶۸۷۲ belonging toGSE۶۹۶۹ superseries) were chosen and combined. A table of Human USP superfamily genes wascollected from UniProt. USP genes with differentially expressed conditions were identified.Results: The analysis results identified ۱۳۲۷ DEGs, including ۹۲۸ downregulated and ۴۴۵upregulated genes. By comparing DEGs with human USPs, five genes (USP۱, USP۶, USP۸,USP۱۳ and USP۱۶) were identified as the final list. The area under the ROC curve (AUC) ofUSP۱, USP۶, USP۸, USP۱۳ and USP۱۶ were ۰.۹۶, ۰.۹۳, ۰.۹۴, ۰.۷۴ and ۰.۸۸, respectively showingthe significant diagnostic values. Conclusion: The results of this study demonstrated that USPsare important in the normal spermatogenesis to a better knowledge of the ubiquitinationmechanism related to teratozoospermia and male infertility.

کلیدواژه ها:

Male infertility ، Teratozoospermia ، USP ، Microarray ، Gene expression