A comparative genomic analysis of clinical and vaccine bacterial isolates using pulsed-field gel electrophoresis

سال انتشار: 1403
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 203

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شناسه ملی سند علمی:

CARSE08_306

تاریخ نمایه سازی: 10 دی 1403

چکیده مقاله:

Considering the circulation of Bordetella pertussis clinical strainsamong populations with high vaccination coverage, it is necessary to have aproper understanding of this bacterium causing whooping cough. Varioustechniques, which are available for studying B. pertussis, can facilitate a propercomparison between different populations. We genotypically analyzed acollection of two vaccine strains used for the production of killed pertussisvaccine during ۲۰۰۰-۲۰۱۴ at Razi Vaccine and Serum Research Institute,Karaj, Iran. Ten clinical and two reference (Tohama ۱ and ۱۸۳۲۳) strains wereused by means of pulsed-field gel electrophoresis (PFGE). The genetic profilesof the vaccine master and working seeds showed no significant changes in thefrequency of fingerprint types in the vaccine strains; also, the homogeneity ofthe profiles was demonstrated. However, the clinical isolates showedheterogeneity in the genetic profiles. In addition, serotyping was performed withmonoclonal antisera to agglutinogens ۲ and ۳. Analysis of the fimbriae showedthat all ten clinical strains expressed Fim۳.

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نویسندگان

Anahita Bahmanjeh

Department of Virology Vaccines, Production and Research Unit, Pasteur Institute of Iran,Karaj, Iran

Samira Zarei

Assistant Professor, Department of Microbiology and Genetics, Kherad Institute of Higher Education, Bushehr, Iran

Morteza Rajabi

Department of Genetics, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Hooman Hanifehpour

Department of Microbiology, Institute of Avicenna Biomedical Sciences, Tehran, Iran

Fatemeh Ashrafi

Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran