Testing the effectiveness of nanoparticle vaccines against lung infections in a murine lung infection model

Klebsiella pneumoniae can cause destructive changes to humanlungs if aspirated. The present study aimed to evaluate the immunogenicity ofthe carriers of Poly lactic-co-glycolic acid (PLGA) and Methoxypoly(ethyleneglycol) Poly(caprolactone) (MPEG-PCL) nanoparticles containing the capsularantigen of Klebsiella pneumoniae K۲O۱ in a model of pulmonary infection inmice as a vaccine candidate. Capsule antigen was extracted fromK.pneumoniae K۲O۱ strain ۱۰۵۳ ATCC ۱۰۰۳۱ and transported with PLGA orMPEG-PCL nanoparticles as a vaccine in an animal model. The results of FTIRand AFM confirmed the presence of antigen functional groups in thenanoparticle structure, and semi-spherical shape of the nanoparticles,respectively. The capsular polysaccharide was also used to evaluate thefebrileness of the designed vaccine candidates based on the rabbits’ pattern,and mortality due to the vaccine candidates in the mice. No fever wasobserved, and no mortality was observed in the mice. According to the results,the vaccine candidates designed to control the cause of pulmonary infectionswere effective in the liver, spleen, and lungs of the animals with the ability toenter the first stage of the clinical trial phase.

کلیدواژه ها:

Klebsiella pneumonia ، nanoparticle ، pulmonary infections ، vaccine