Isolation of Bioactive Peptides from Rocky Shore Crab, Grapsus Albolineatus, Protein Hydrolysate with Cytotoxic Activity against ۴T۱ Cell Line

Aims: Breast cancer is the most common cancer in women in several countries. Bioactive peptides have demonstrated their cytotoxic potential in numerous cancer cell lines. In the search for novel bioactive peptides for pharmacological properties, crab is noncommercial protein-rich species. Using enzymatic hydrolysis is an efficient way to recover potent bioactive peptides from marine sources. Materials and Methods: The aim of this study was to isolate fractions from rocky shore crab hydrolysate with desired molecular weight by ultrafiltration and investigate their cytotoxic activities. Four fractions (>۳۰kDa, ۱۰-۳۰kDa, ۳-۱۰kDa and <۳kDa) were evaluated for cytotoxic activity against a ۴T۱ cell line by MTT assay. Findings: The MTT assay showed that although all fractions from the crab hydrolysate showed some activity, the low molecular weight samples (۳-۱۰kDa and <۳kDa) were more effective than high molecular weight fractions (>۳۰kDa and ۱۰-۳۰kDa) while the ۳-۱۰kDa fraction proved to be the most effective. The low molecular weight fractions significantly reduced the viability of the ۴T۱ cell lines in a dose-dependent manner upon ۲۴ and ۴۸h. The results were recorded in IC۵۰ values of about ۰.۴۰±۰.۰۶۳mg mL-۱ for <۳ and ۰.۲۵±۰.۰۲۶mg mL-۱ for ۳-۱۰kDa fractions. Conclusion: Peptide fractions were isolated from the protein hydrolysate of the rocky shore crab Grapsus albolineatus are able to inhibit cancer cells and can be considered as a novel agent in nutraceutical and pharmaceutical ingredient applications.

کلیدواژه ها:

Rocky Shore Crab ، Bioactive Peptide ، Cytotoxic Activity ، ۴T۱ Cell line