Elucidation of the Molecular Interaction between GreenSynthesized Carbon Dots and Hemoglobin by FluorescenceSpectroscopy

Fluorescent Carbon Dots (CDs) have gained paramount interest in chemical biological and biomedicalfields. In this work, in vitro molecular interaction of green synthesized CDs (g-CDs) with humanhemoglobin (HHb) have been systematically studied by fluorescence spectroscopy technique underphysiological conditions. It was found that the intrinsic fluorescence of g-CDs was quenched by theaddition of HHb and the quenching mechanism was considered as static quenching by formation of aHHb-(g-CDs) complex. The binding constant was about ۱۰۸ M−۱ at room temperature. The values of thecalculated thermodynamic parameters (ΔH۰ < ۰ and ΔS۰ > ۰) suggested that both hydrogen bonds and thehydrophobic interactions were involved in the binding process.

کلیدواژه ها:

Carbon dots ، Green chemistry ، Interaction ، Hemoglobin ، Fluorescence quenching