Using Natural-Product-Based Treatments, Such as Polymer loaded with Ginger for the Management of Osteochondral Disorder

This study reported the biological changes occurring after γ-irradiation of in vivo rat model and the osteochondral protective effect of Gelatine-Chitosan-Ginger (GEL-CH-GING). The results showed that Electron Paramagnetic Resonance (EPR) Spectroscopy of GEL-CH-GING showed two paramagnetic centres which correspond to g=۲.۱۹ and g= ۲.۰۰۲. The Fourier transform infrared spectroscopy (FTIR) analyses revealed an increase in peak intensity at C–H chains, as well as, C=O carbonyl groups. The X-ray diffraction (XRD) analysis showed no change of crystallinity. After gamma ray exposure, the rat groups have received an osteochondral defect and then were treated with GEL-CH-GING composite. Sixty days post-surgery, a significant reduction in thiobarbituric acid-reactive compounds (TBARs) was seen when compared to non-implanted rat group. Concerning oxidative stress status, GEL-CH-GIN significantly improved Superoxide Dismutase (SOD) ۷۶ nmol/l, Catalase (CAT) ۰.۷۹ nmol/l, and Glutathione Peroxidase (GPx) ۱.۷۷ nmol/l activities in osteochondral tissue. Regarding the histomorphometric parameters of cartilaginous tissue (nCg.Th, µm), (cCg.Th, µm), (Cg.Th, µm), irradiated-GEL-CH-GIN group showed a significant increase as compared to irradiated group with ۱۱۶, ۷۴ and  ۱۸۸ µm, respectively (p<۰.۰۱). The microanalysis showed a high percentage of O and C in the regenertaed osteochondral tissue and indicated the deposition of novel collagen matrix. The biomechanical behaviour showed a significantly enhanced hardness measurement (۱.۷۳±۰ .۰۲۹ VH, p<۰.۰۵) when compared with that of irradiated group Biochemical markers suggested an osteocartilage repair capacity.  In fact, the levels of IL-۱β, IL-۶, TNF-α and VEGF in the implanted rat with GEL-CH-GING composite exhibited ۵۱±۳.۴۸, ۳۰.۰۵±۵.۱۸, ۶۵.۱۲±۴.۳۳ and ۴۰.۴۲±۳.۳۲ ng/l, respectively. Our findings suggested that GEL-CH-GING composite might have promising potential applications for cartilage healing.

کلیدواژه ها:

Ginger ، γ-irradiation ، Biomaterial ، oxidative stress ، osteochondral defect