Magnetic graphene oxide increases the biocompatibility and nuclear factor erythroid ۲-related factor ۲ antioxidant of human cumulus cells: A lab-trial study

Background: Although assisted reproductive technology has been improved, the success rate is only ۳۰%. Since the interaction between oocytes and cumulus cells (CCs) is necessary for the formation of a fertile oocyte, increasing the survival rate of CCs can improve the function of oocytes in infertile women. Objective: This study aimed to investigate the effects of magnetic graphene oxide (MGO) nanocomposite on the biocompatibility and antioxidant activity of human CCs. Materials and Methods: In this lab-trial study, from July ۲۰۲۱-۲۰۲۳ human CCs were collected from ۳۷ women aged ۲۰-۳۷ yr and cultured in a medium containing Dulbecco’s Modified Eagle’s/F۱۲, fetal bovine serum (۱۰%), and penicillin-streptomycin (۱%). Then CCs were treated with increasing concentrations of nano-MGO for ۲۴, ۴۸, and ۷۲ hr (۳[۴, ۵-dimethylthiazole-۲-yl]-۲, ۵-diphenyltetrazolium bromide) assay and flow cytometry technique were used to compare the survival rate and apoptosis of CCs before and after treatment. Western blot test was used for expressing nuclear factor erythroid ۲-related factor ۲ (Nrf۲) antioxidant in ۲ groups. Results: The results of the present study showed that treatment with MGO increased the viability of CCs at a concentration of ۵۰ µg/ml after ۴۸ hr (p > ۰.۰۱). At higher doses (۱۰۰ µg/ml) MGO decreased the survival rate of CCs (p > ۰.۰۵). Also, treatment with MGO at a concentration of ۵۰ µg/ml increased the expression level of antioxidant protein Nrf۲ in human CCs. Conclusion: Our results highlight the use of MGO in a new strategy that improves CCs viability and secretion of antioxidant protein Nrf۲, thereby potentially increasing in vitro fertilization outcomes.   This article has been extracted from Ph.D. Thesis. (Fahimeh Kabiri)

کلیدواژه ها:

Magnetic graphene oxide ، Cumulus cells ، Fertility ، Nrf-۲ ، Western blot. ، اکسید گرافن مغناطیسی ، سلول های کومولوسی ، باروری ، Nrf۲ ، وسترن بلات.