Diagnosis of hydatidiform moles using p۵۷ immunohistochemistry and chromogenic insitu hybridization: A retrospective study

Background: Chromogenic insitu hybridization (CISH) and immunohistochemistry analysis for p۵۷ are ancillary studies discriminating partial hydatidiform mole (PHM), complete hydatidiform mole (CHM), and non-molar hydropic abortion (HA). Objective: It aimed to study CISH with a probe to chromosome ۱۷ (CISH۱۷) and chromosome ۲ (CISH۲) discriminating chromosomal ploidy of PHM, CHM, and HA; in addition, their surrogacy value in the evaluation of triploid and diploid in product of conception specimens (POCs) was evaluated. Materials and Methods: ۴۴ statistically significant POCs were selected retrospectively. The Kappa agreement coefficients, sensitivity, specificity, and accuracy with ۹۵% confidence interval (۹۵% CI) were reported. Results: PHM, CHM, and HA were diagnosed to be ۲۳, ۱۷, and ۳ cases based on both CISH۲ and CISH۱۷ resulting in their complete discrimination between PHM and HA (۲۳ vs. ۳). The Kappa agreement coefficient was ۹۵.۴% (p < ۰.۰۰۱) when diagnosing the PHM (۲۳), CHM (۲۰), and HA (۱). In addition, the accuracy, sensitivity, and specificity were ۹۵.۲۶% (۹۵% CI: ۸۴.۲۵-۹۹.۳۸), ۱۰۰% (۹۵% CI: ۸۵.۱۸-۱۰۰), and ۹۵% (۹۵% CI: ۷۶.۱۸-۹۹.۸۸), respectively. The power analysis on CISH۲ and CISH۱۷ tests discriminating between triploid and diploid in POCs was estimated to be ۱۰۰%. Conclusion: Based on the current finding, CISH۲ and CISH۱۷ enjoyed perfect agreement in diagnosing chromosomal ploidy; in addition, their absolute power discriminating between triploid and diploid revealed that they could be used as surrogate markers for ploidy. Prospective studies on fresh specimens are suggested comparing the CISH method's accuracy with flow cytometry karyotyping and fluorescence in situ hybridization. This article has been extracted from M.D. Thesis. (Tara Taheri)

کلیدواژه ها:

Hydatidiform mole ، CISH protein ، Human ، Pathology ، Clinical. ، هیداتی فرم مول ، پروتئین CISH ، انسان ، آسیب شناسی ، بالینی.