The effect of eight weeks of resistance training with spirulina platensis supplementation on the RAGs/Rheb/mTORC/S۶K pathway in male rat kidneys

Background: Resistance training and protein supplementation are known to increase protein synthesis and hypertrophy, primarily through the activation of the mTORC۱ signaling pathway. However, mTORC۱ activation in the kidneys can potentially lead to kidney disease. This study investigates the effects of eight weeks of resistance training combined with Spirulina platensis supplementation on the RAGs/Rheb/mTOR/S۶K pathway in male rat kidneys. Methods: In this study, ۳۲ male Sprague-Dawley rats were divided into four groups: control (Co; n = ۸), Spirulina platensis (SP; n = ۸), resistance training (RE; n = ۸), and Spirulina platensis + resistance training (SP+RE; n = ۸). The resistance training group engaged in five sessions per week over eight weeks. Spirulina was administered at a dosage of ۲۰۰ mg/kg/day to the supplement and SP+RE groups. Gene expression was analyzed using real-time PCR following the last training session. Results: The mTOR gene expression significantly increased in the SP group (p = ۰.۰۱), while no significant changes were observed in the RE and SP+RE groups. Rheb gene expression did not show significant changes across any groups. Significant changes were noted in the RAGs gene in the SP group (p = ۰.۰۰۱), RE group (p = ۰.۰۴۷), and SP+RE group (p = ۰.۰۲۵). The S۶K gene showed significant changes in the SP group (p = ۰.۰۱) but not in the other groups. Conclusion: Spirulina supplementation may activate the mTORC۱ signaling pathway in the kidneys, potentially contributing to kidney disease progression. However, combined resistance training and Spirulina supplementation did not show changes in mTORC۱ expression, suggesting that this combination might prevent further kidney tissue damage in athletes.

کلیدواژه ها:

Resistance training ، Arthrospira platensis ، kidney ، mTORc۱ ، RAGs ، Rheb protein ، rat ، P۷۰S۶K