Evaluation of Crucial Cell Junction Genes in Endometriosis

Background: Endometriosis is a condition in which the endometrium,the layer of tissue that normally covers the inside ofthe uterus, grows outside of it. Tight junctions are regulated intheir molecular composition ultrastructure, and function by intracellularscaffolding proteins and the cytoskeleton; such regulationserves normal, physiologic adaptation but also occurs innumerous diseases. It has however, become increasingly apparentthat the tight junction has a vital role in maintaining cell tocell integrity and that the loss of cohesion of the structure canlead to endometriosis. The aim of the study was to assess tightjunction genes functions in the context of endometrial biologywith the usage of RT- PCR.Materials and Methods: This research evaluated the expressionof cell junctions genes of desmoglein-۱, E-cadherin andzona occludin-۱ as reliable reference genes in eutopic and ectopicendometrial tissue specimens obtained during standardsurgery of women of reproductive age.Results: The candidate gene expression altered in groups incompared with control group.Conclusion: We describe a genetic basis for endometriosis andprovide strong evidence for the existence of the role of tightjunction abnormality in endometriosis patients.

کلیدواژه ها:

Endometriosis ، Cell Junction ، E-Cadherin ، Desmoglein-۱ ، Zona Occludin-۱