Alteration in Expression of Genes Involved in The Initial Steps of Steroidogenesis in Mice Abdominal AdiposeTissue by Dietary Saturated and Trans Fatty Acids

Background: Adipose tissue (AT) constitutes an importantsite for steroid hormone metabolism. It seems that eating habitssuch as oils with higher content of saturated or trans fattyacids (FA) are the main causes of subfertility. However, to ourknowledge, the question of whether consumption these FA affectssteroidogenesis in AT has not been addressed. The currentstudy was designed to determine whether dietary saturated ortrans-FA influences the expression of two rate-limiting factorsof steroidogenesis, i.e., steroidogenic acute regulatory protein(Star) and cytochrome p۴۵۰ (CYP۱۱A۱) in male mice AT.Materials and Methods: Mature male mice (۸ weeks) weredivided into the fat diet (FD) (n=۱۵) and control (n=۱۴) groups.FD group fed tallow for ۲ months and abdominal AT sampleswere collected and performed quantitative RT-PCR.Results: The levels of Star transcripts in FD group were significantlylower compared to the control group. The levels ofCYP۱۱A۱ in FD group significant higher compared to the Cgroup (P<۰.۰۵).Conclusion: A significant increase in the mRNA abundanceof CYP۱۱A۱ indicates that AT expresses the CYP۱۱A۱ in thepathway from cholesterol to active steroid hormones and denova steroid synthesis in AT. The present results demonstrate the stimulatory effects of saturated or trans FA on the initialsteps of steroidogenesis in AT as well as hormonal imbalance inmale which warrants further studies.

کلیدواژه ها:

Star ، CYP۱۱A۱ ، Adipose Tissue ، Steroidogenic