Effect of Vitrification on Expression of Apoptotic Genes in Mouse Metaphase II Oocytes following Cryotop Method

Background: Vitrification is an effective approach for cryopreservationof human oocyte and embryos. The aim of thisstudy was to investigate the vitrification effect on Bax and Bcl-۲ expression level in mouse metaphase II (MII) oocytes.Materials and Methods: The survival and fertilization rate ofMII mouse oocytes was assessed following vitrification by cryotop.Oocytes were randomly selected and distributed amongstthe five experimental groups (control, docetaxel, docetaxel +VS (Vitrification solution), docetaxel + Vitrification, and vitrification).Then, vitrification method effect on the expressionof Bax and Bcl-۲ genes were determined in vitrified-warmedoocytes by real-time RT-PCR. Each group was compared withthe control. Data were analyzed with ANOVA using Graph Padand SPSS version ۲۱ software.Results: There were significant differences between Bax geneexpression level in the control group with the docetaxel andvitrification groups (P<۰.۰۵). Bcl-۲ gene expression level wassignificantly high in the vitrification group (vitrification + Docetaxel)and non-vitrified group (Docetaxel + VS) (P<۰.۰۱).Conclusion: This study indicated that the vitrification of mouseMII oocytes can increase Bax and Bcl-۲ genes expression.

کلیدواژه ها:

Vitrification ، Oocytes ، Docetaxel ، Bax ، Bcl-۲