The Study of Relation Between CGG Repeats in FMR۱ Gene and Molecular Genotye of Ovary in PCO Women withTP-pcr Technique

Fragile X syndrome is recognized as a retardation that is dependenton chromosome X.In women this syndrome is accompaniedwith different degrees of mental retardation. TheFMR۱ gene was identified as the gene responsible for fragileXsyndrome in ۱۹۹۱ and it plays a key role in ovaries functionregardless of nervous effects. FMR۱ mutation is the result ofan increase in the number of CGG codon repetition. Polycysticovarian syndrome (pco) is one of the most common endocrinologydisordersin women , and the most common cause ofinfertility due to an absence in ovulation.In numerous studiesconduct in countries other than Iran in the past , a close connectionbetween an increase in CGG repetition in FMR۱ geneand ovaries disorder was observed. Due to the importance ofthis disorder and the difference between gene pools in Iran andother countries, the aim of this study is to analyze the relation between the number of triple CGG repetitions in FMR۱geneand poly cystic ovarian syndrome in women who visited Yashospital with this syndrome between the years ۱۳۹۵-۱۳۹۶ .Thus in order to conduct this study , we randomly selected ۵۰patients , extracted their DNa via their blood and determinedthe repetition number of CGG , using TP-PCR method. In thistechnique we used Forward primer that was marked witth FAM(a florescent material) . this technique determines whether ornot the desired band exist , as well as the exact number of triplerepetitions.In conclusion , after analyzing the obtained DNAsequences , only ۷ patients with higher than normal CGG repetitionwere found and this limited number can not prove a rerliableand solid connection between CGG repetition numbers andpoly cystic ovarian syndrome in Iran`s big population . Dueto the vast vriety in Iranian ethnicities , and the richness anddifference in Iranian gene pool , it is possible to achieve betterresults will the help of professors and new techniques in thefuture.