Effects of L-Carnitine on In Vitro Maturation and Developmental Potential of Oocytes Obtained from TransplantedMouse Ovarian Tissues

Background: Ovarian tissue transplantation is an emergingtechnology for fertility preservation. In addition, in vitro maturationof oocytes retrieved from grafted ovaries may overcomethe fertility defects in some cases. The objective of this studywas to evaluate the potential of using L-carnitine (LC) as anantioxidant to improve developmental potential of oocytes obtainedfrom grafted ovarian tissues.Materials and Methods: NMRI mice were divided into fourgroups: control (non-grafted), transplant(autograft withouttreatment), saline group (autograft +saline), LC group (autograft+ LC). ۶- weeks- old mice were ovariectomized and leftovaries were transplanted into the back muscle tissue. LC (۱۵۰mg/Kg) was injected intraperitoneally one day before surgicaloperation and repeated until one week after grafting. ۳ weekslater, ovarian grafts were recovered and oocytes were harvestedfor in vitro maturation (IVM), in vitro fertilization (IVF) and invitro development (IVD).Results: Our results indicated that the number of retrieved immatureoocytes as well as successful IVM, IVF and IVD intransplanted groups was significantly lower than control group.(P<۰.۰۵). All transplanted ovaries contained some oocytes thatsurvived following IVM, IVF and IVD, and no significant differencewas seen between grafted groups.Conclusion: Our study demonstrates that L-carnitine alone didnot show any negative effect on further development of oocytes.It seems that usage of LC in combination with a scaffoldcould improve autotransplantation results and more studies areneeded in this area.

کلیدواژه ها:

Transplantation ، L-carnitine ، In Vitro Maturation