The Study of The Quality of Sperm DNA in Semen Samples Processed in Three Ways: Passing Through The Cumulus Column, Concentration Centrifugation and Incubation with Supernatant of Mesenchymal Stem Cells Derived from Fat Tissue

Background: Recently, the measurement of the common parametersof semen for evaluation of sperm quality is less importantbecause these consideration cannot guarantee the absenceof male infertility factors. Nowadays Clinical evidence suggeststhat DNA damage to sperm DNA be considered a disadvantagefor reproductive results. Therefore, further studies are needed todetermine a semen preparation method with minimal damage tothe DNA of the sperm nucleus and its effect on reproduction.Materials and Methods: In the present study, of infertile mensemen samples with asthenoteratozoospermia who referring toinfertility treatment center were processed in three ways: DGC,cumulus column, and SPAS. The results of sperm parametersand sperm count DNA fragmentation before and after the processwere statistically analyzed.Results: The results showed that the number of natural morphologysperms isolated during the SPAS and the cumulus pillwas significantly more than the corresponding population in theDGC group. In addition, although all three methods have thesame ability to increase total sperm movement and the numberof recovered sperm, in the field of forward movement and DNAfragmentation, the SPAS method functioned more efficiently(P≤۰.۰۵).Conclusion: Considering that it has been shown that the spermcapacity is increased during the SPAS method. On However,the rearrangement of sperm chromatin by reducing the disulfidebridges and providing the possibility of re-histone overcapacity, causes a significant reduction in DNA fragmentationwhich is not expected in this way.

کلیدواژه ها:

SPAS ، DGC ، Cumulus Pill ، DNA Fragmentation