Growth of Human Endometrial Cells on Electrospun Polycaprolactone/Polydimethylsiloxane Nanofibrous Scaffoldfor Uterine Tissue Engineering

Background: In this study, a novel PCL/PDMS scaffold withimproved mechanical properties and controllable porous structurewas prepared through electro spinning method. The scaffoldbiocompatibility was further studied, using human foreskinfibroblast. MTT assay results indicated that human endometrialcells grew well on the PCL/PDMS scaffold with a notable parallelarrangement. Although further studies, such as extracellularmatrix production and functional gene expression, arenecessary to confirm the proper biocompatibility of the PCL/PDMS scaffolds, the results indicate that the PCL/PDMS scaffoldcould be a new suitable scaffold for uterine tissue engineeringMaterials and Methods: Polycaprolactonel (PCL) and polydimethylsiloxanevinyltherminated were purchased from Sigma-Aldrich® and Trifluoroacetic acid (TFA) were purchased fromMerck® companies. endometrial cells were extracted from normalhuman uterine tissue samplesResults: Endometrium cell were cultured in DMEM. a surfacephenotype (CD۹۰+, CD۱۰۵+, CD۱۴۶+, and CD۴۵) distinguishingthem from mesenchymal stem cells (MSCs). After sterilizationunder UV light for ۲ h, the electrospun fibers were placedin ۲۴-well plates and were seeded with endometrial cells at adensity of ۱۰,۰۰۰ cells/well. The cells were incubated in a humidifiedstandard incubator at ۳۷ °C with ۵% CO۲. Cells wereharvested at days ۳, ۷, and ۱۰ for growth, phenotypical evaluationand MTT test. By triplicate assay the activity and survivalof the cells on nanofibers were calculated as a percentage ofsamples to control ratio.Conclusion: The random, aligned scaffolds were developed bythe electrospinning technique, and their morphological microstructureswere investigated by SEM. SEM micrographs of thescaffolds were obtained before seeding of the cells. The humanfibroblasts showed a well growth and replication on the scaffolds.The endometrial cells on all four types of scaffolds werefound to spread well and the cells were grown on the scaffold.Also, significant difference in cell growth and replication onthe scaffold and control samples indicates the lack of toxicityof the wizard for the scaffold adaptation. These findings couldbe used in endometrial tissue engineering as the first step foruterine tissue engineering. We planned to evaluate a fertilizedegg response on this novel scaffold.

کلیدواژه ها:

Endometrium ، Nano Fiber ، Polycaprolactone/Polydimethylsiloxane ، Scaffold ، Tissue Engineering