۶-Gingerol Improves Human Freezing Thawing Process

Background: Cryopreservation of human sperm associatedwith an increase in reactive oxygen species (ROS) which leadto lipid peroxidation of sperm membrane. Natural antioxidantsimprove the quality and metabolic activity of sperm during thecryopreservation. Ginger (Zingiberofficinale Roscoe, Zingiberaceae)contains volatile oil rich pungent principiesShogaols,zingerone, and gingerols) that exhibited substantial antioxidantactivities. In this study we evaluated the effect of gingerol asan antioxidant in sperm cryopreservation medium on differentsperm parameters during the freezing thawing process.Materials and Methods: Semen were collected from ۲۰ normospermicmen referring to the Royan Institute and dividedinto three groups; Fresh group, control freezing group andfreezing with ۶-gingerol (final concentration of ۱۰μM) group.Finally, sperm motility the levels of malondialdehyde (MDA)and acrosome intact were evaluated in all groups.Results: Evaluation of sperm motility was performed withcomputer-assisted-semen analysis (CASA). Total motility andprogressive motility was significantly Lowe in freezing group(۲۹.۹۰% ± ۲.۱۳% and ۳۰.۰۳% ± ۲.۸۲ resp) compared to freshgroup (۶۱.۲۷% ± ۲.۹۵). However, MDA levels in the freezinggroup were higher than fresh group (P<۰.۰۵).There was no significantdifference between freezing and freezing + Gingerol insperm motility and MDA levels. Acrosome intact were evaluatedby hypo osmotic swelling test (HOST). Acrosome intact infresh and freezing + Gingerol groups were significantly higher(۸۴.۴۵% ± ۱.۹۴% and ۶۳.۵۰% ± ۱.۴۱% respectively) than freezinggroup (۵۳.۲۵% ± ۱.۵۱).Conclusion: Accordingly, we concluded that although Gingerolimproved sperm viability, it did not show positive effecton MDA levels and motility. It seems that addition of Gingerolto freezing medium can improve sperm freezing condition.

کلیدواژه ها:

Cryopreservation ، Sperm Quality ، Gingerol