The Comparative Survey of Antioxidant Compositions on MDA, TAC and SOD of Ram Epididymal Sperm

Background: The anti-oxidant system has been described asa defense functioning mechanism against lipid peroxidationin semen, and is important in maintaining sperm motility andviability. Mammalian sperm membranes incorporate manyunsaturated fatty acids and are susceptible to lipid peroxidation(LPO) in the presence of reactive oxygen species (ROS),leading to decreased sperm quality. Ram sperm are subjected toextreme oxidative stress during their preservation in medium.Lipid peroxidation was measured in terms of malondialdehyde(MDA), total antioxidant capacity (TAC) and superoxide dismutase(SOD) levels in semen.Materials and Methods: In this study, at overall, sperms werecollected from ۲۰ pairs of ram testicles. Collection was carriedout in slaughtered animals. Sample processing was performedin a walk-in fridge (۵°C) immediately after its arrival to laboratory.Then tail of epididymis were fixed between two fingers.Sperm were collected performing several incisions in the caudalepididymis with a surgical blade. Five levels of cysteine(۱-۲-۴-۸-۱۰ mM), ۴ levels of L-carnitine (۲-۴-۸-۱۰), ۴ levelsof Glutathione were added into human tubal fluid containingsperms (۴۰×۱۰۶ sperm/ml), with ۱۰%, bovine serum albuminand were kept for ۱۲ hours at ۵ºC then at ۱ and ۱۲ hrs, theamount of Total anti-oxidant capacity (TAC), Superoxide dismutase(SOD) and Malondialdehyde (MDA) were evaluatedwith spectrophotometry.Results: Data were analyzed using SPSS ۲۵ statistical package.Multiple comparisons by post-hoc test such as tukey and tamhanewere performed to determine statistical differences amonggroups. Results were considered significant at P<۰.۰۵ for alltests. The results showed that Glutathione at ۲-۸-۱۰ Mm levelsin contrast of control had the best effect on TAC increases andreduction of MDA.Conclusion: Between amino acids that as antioxidant addedto culture medium contain of ram epididymal sperm cells, glutathionehad the best effect on sperm TAC and MDA levels during۱۲ hrs storage at ۵ºC

کلیدواژه ها:

MDA ، TAC ، SOD ، Ram ، Sperm