The Effect of Hydroalcoholic Extract of Nettle Leaves on The Occurrence of Miosis in NMRI Mice

Background: The Oocyte is a unique cell in woman’s body, notonly in its special structure and function, but it is the only cellthat undergoes meiosis. in vitro maturation (IVM) is an attrac tive treatment alternative for women with various forms of infertilitywithout recourse to ovarian stimulation.Approximately۲,۰۰۰ healthy infants have been born following immature oocyteretrieval and IVM. Therefore, this study has been conductedwith the aim of the efficacy of hydroalcoholic hackberryleave extract on in vitro maturation of premature mice ovum.Materials and Methods: In this study, female mice aged ۶-۸week, were used. ۴۸ hours after injecting PMSG hormone,when ovaries aren’t still mature, mice were killed by cervicaldislocation and ovaries were removed. The hackberry extractwas added to the experimental groups by the concentrations of۲۵۰, ۵۰۰, ۱۰۰۰, ۲۰۰۰ mg/ml and was incubated in CO۲ incubatorfor ۲۴h. The stages of ovum maturity were examined andSPSS software was used to analyze of data.Results: The average percentage of MI oocytes in the experimentalgroup with a concentration of ۲۰۰۰ mg/ml showed a significantincrease and decreased in other experimental groups.The average percentage of MII oocytes in the experimentalgroup with the highest concentration of ۵۰۰ micrograms per mgshowed a significant increase compared to the control group. Itsrate increased in all experimental groups, but decreased significantlybetween the control and experimental groups at a concentrationof ۲۰۰۰ mg/ml.Conclusion: In general, the results of this study showed thatthere were more maturational concentrations and the oocytematuration rate should be reduced at high concentrations.

کلیدواژه ها:

Oocyte ، In Vitro Maturation ، Hydroalcoholic Hackberry ، MI Oocytes ، MII Oocytes