Intrauterine Administration of Treated PBMC Prior Frozen/Thawed Embryo Transfer Improves Pregnancy Outcomes in Patients with Repeated Implantation Failures

Background: Implantation success is mainly dependent onlocal immune-tolerance mechanisms involving a spectrum ofcytokines, interleukines and growth factors.The aim of thisstudy was to evaluate clinically the effectiveness of intrauterineadministration of treated PBMC prior frozen/thawed embryotransfer for RIF patients undergoing IVF program.Materials and Methods: This study is a randomized clinicaltrial, including ۲۵۰ couples (PBMC-test, n= ۱۲۲ and Control,n= ۱۲۸) who being part of Frozen-thawed embryo transfercycles. In tread group a blood sample is scheduled five daysbefore frozen-thawed embryo transfer and PBMC was isolatedusing a separation protocol based on Ficoll. PBMC is well preparedafter a culture for ۴۸-۷۲ h. Then, ۰.۴ml of cultured PBMCtransferred to the patient of PBMC group, in uterus ۲-۳ daysbefore embryo transfer.Results: Clinical pregnancy rate was increase from ۲۳.۴%for control group to ۳۴.۴% for PBMC group but this differentwasn’t statistically significant (P=۰.۰۷, chi-square). Whenwe limited our analysis to patient with ≥۳ RIF (n=۱۳۸), therewere a significant difference in clinical pregnancy rate (۳۸.۶%vs ۱۹.۸%) between PBMC and control groups (P=۰.۰۲, chisquare).Conclusion: The findings of this study indicate that the use ofPBMC can be an effective treatment for infertile patients withRIF.

کلیدواژه ها:

Peripheral Blood Mononuclear Cells ، Corticotropin-Releasing Hormone ، In Vitro Fertilization ، Embryo Implantation ، Pregnancy Rates