The Pore Size Effect of ECM-Modified Alginate for Human Embryonic Stem Cell-Derived RPE Attachment

Background: Alginates are biocompatibility and biodegradabilitypolysaccharide which can form hydrogel in the presenceof divalent cations such as Ca۲+. Without any modification,alginate is not suitable for cell attachment. Also, the porosityplays an important role. Porosity of alginate, should be suitablefor cell growth, attachment and migration. Also it’s important toinfiltration of oxygen and the movement of nutrients and metabolicwaste and prevent cell lack from the scaffold.Materials and Methods: In this study, a thin layer of alginatewas prepared by three different concentrations, ۰.۶%, ۱.۵% and۳% (w/v) . Next, sodium alginate layer was gelled by addinga CaCl۲ solution. After that, the ECM was added to the alginateand dried at room temperature. Finally, the human stemcell-derived RPE were cultured on ECM-Alginate and the cellmorphology was assessed.Results: ۱۰ days later, the cell morphology and expansion improvedmost effectively within ۱.۵% alginate gel comparedwith ۰.۶% and ۳% alginates.Conclusion: In this study, we designed alginate layer mimickingBruch’s membrane for hESC-RPE. Our results showed thatalginate porosity could affect the attachment and expansion ofRPE. Internal pore size of alginate gel increased with decreasingalginate concentration and increase in alginate gel porosity,that augmented the secretion of proteins and solutes fromRPE cells, enhanced greatly cell attachment and maintenance.Therefore, the alginate layer with define pore size could be effectivefor RPE attachment.

کلیدواژه ها:

RPE ، Alginate ، Poe Size