Effects of Autologous Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells on Arterial Blood Gas Pressure in Experimental Model of Acute Respiratory Distress Syndrome in Sheep

Background: Acute respiratory distress syndrome (ARDS)is alung disorder that causes death in human and Animals. Due tothe high prevalence of pulmonary disease worldwide, developingpreventive therapies to reduce lung disease are important.There is no particular therapeutic drug for ARDS. For this reason,achieving a new therapeutic approach, such as stem celltherapy, is a necessary requirement. The use of mesenchymalstem cells for the treatment of pulmonary injuries has become apromising therapeutic approach.Materials and Methods: Ten male Shall sheep were used afterrandom placement into two groups. In the treatment groupbone marrow samples were collected and BM-MSCs isolatedand amplified. Then an experimental model of ARDS was inducedby intratracheal injection of LPS. Radiograph imagesperformed before and after injection of LPS. After confirminginflammation and ۵۰×۱۰۶ cells of BM-MSCs were transplantedin the treatment group as autolug. An arterial blood sample wasused for analysis of blood gases. The Arterial samples werecollected from the ear artery in the before and ۳,۶,۱۲,۲۴,۴۸,۷۲and۱۶۸ hours after BM-MSC transplantation or PBS injectionand the sample were processed with Blood Gas Analyzer, immediately.Blood gas analyzer measured pH, the partial pressureof oxygen(PO۲), the partial pressure of carbon dioxide(PCO۲)and bicarbonate(HCO۳)on arterial blood samples.Results: The results showed that stem cell transplantation couldmodulate hypoxemia in inflammation, unlike the control group.Arterial blood gas analysis was improved in lung injury followingMSC treatment. The survey of the PO۲ showed a significantdecrease in the two groups after creating an experimental model of ARDS. But after the BM-MSCs transplantation PO۲increased, so that it was significant at times۲۴,۴۸,۷۲ and ۱۶۸ hcomparison inflammation time. Also, decrement in PCO۲ occurredafter the BM-MSCs transplantation, so that the reductionwas significant at times of ۲۴,۴۸,۷۲ h compared with baseline.Conclusion: The results indicated that BM-MSCs could improvearterial blood gas and play a significant role in the repairof ARDS.

کلیدواژه ها:

BM-MSCs ، ARDS ، Arterial Blood Gases ، Sheep