Development of Functional Heart Ventricle Using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

Cardiovascular diseases (such as myocardial infarction and coronaryheart disease) are reported as the most pernicious onesin the world. Current animal models cannot be reliable modelfor human heart performance due to noticeable differences ingenetics, disease etiology and physiology, which limit theirutility. Moreover, laboratory studies were not able to evaluatevolumetric performance of the heart. In this study, we aimed tofabricate a multilayer three dimensional ventricular constructsimilar to anisotropic orientation and function of native myocardium.This smallest functional target has thickness of ۱.۱mm. Scaffolds with ۱۱ layers, thickness of ۰.۱ mm and lengthof ۱ cm for each layer were designed to resemble the model.Cardiomyocytes were autonomously self-assembled into architecturemimicking the native leftventricle. Scaffold propertieswere optimized to achieve high cellular attachment and properorientation on the scaffold. A polydimethylsiloxane bioreactorwas designed and fabricated to culture cell-laden scaffold. Thecell-laden scaffold was then wrapped around a printed coneand cultivated for ۷ days. The cell viability, alignment and contractilitywere evaluated for designed ventricular structure. Weexpect that multi-layer cardiomyocytes with similar orientationshould be capable of synchronously twisting and contractingas the native left ventricle. The proposed research not only addressesurgent health problems in the world, but also helps developa novel technology for one of the most highly demandedmedical device markets. This artificial left ventricle would bean ideal platform for drug testing and pave the way for developingmore complex heart chambers.

کلیدواژه ها:

hiPSC-Derived Cardiomyocytes ، Left-Ventricle ، Cardiac Tissue Engineering ، In Vitro Model