Reconstruction of the miRNA-mRNA regulatory network specifically in the remission and relapse phase of people with multiple sclerosis

Multiple sclerosis (MS) is a chronic, unpredictable disease of the central nervous systemthat is known for its periods of remission and relapse. The molecular mechanisms underlying thesephases are complex and not fully understood. miRNAs are small non-coding RNAs that regulate geneexpression at the post-transcriptional level. This study aims to elucidate these mechanisms byreconstructing the miRNA-mRNA regulatory network specific to these phases of MS. GSE۴۱۸۹۰ wasdownloaded from GEO database. In this dataset, the gene expression data was taken from the peripheralblood leukocytes. The difference in gene expression in the relapse and remission groups was analyzedcompared to the control group. Then, using the miRTarBase database, we identified the microRNAsrelated to the mRNAs of each group and reconstructed the regulatory network using Cytoscapesoftware. Our results showed H۲AC۱۸, H۲BC۲۱ and H۳C۱۰, hsa-miR-۳۴a-۵p, hsa-miR-۱۹۲-۵p andhsa-miR-۵۶۹۳ have regulatory role in relapse network and MCM۶, SCD and GEN۱, hsa-miR-۱۲۴-۳p,hsa-miR-۱۹۲-۵p and hsa-let-۷b-۵p have regulatory role in remission network. In this study we identifiedkey miRNAs and mRNAs that may serve as potential biomarkers or therapeutic targets in MS.

کلیدواژه ها:

Relapse ، remission ، multiple sclerosis ، miRNA