G-quadruplex structures in ring finger protein ۱ (RING۱) gene sequence

Recent studies have shown that RING۱ is overexpressed in various types of human cancers,including lymphoma, non-small cell lung cancer, and prostate and liver cancers [۱]. The Ring fingerprotein۱ (RING۱), an essential component of the poly-comb family of proteins, plays vital roles in thetumorigenesis of various cancer types [۲]. However, further research is required to determine RING۱expression and prognostic value in some cancers. Therefore, regulating its gene expression is importantfor disease treatment. One of the ways to control the expression of this gene is to induce G-quadruplexstructures, which act as a barrier to the gene expression apparatus [۳]. Interestingly, there is a databasethat lists quadruplex-forming G-rich sequences (QGRS) (http://tubic.tju.edu.cn/greglist/) [۴]. Thesequence of the RING۱ gene was obtained from GenBank at NCBI. We used a web-based server, QGRSMapper, which predicts quadruplex-forming G-rich sequences (QGRS) in nucleic acid sequences(http://bioinformatics.ramapo.edu/QGRS/) [۵]. RING۱ was a large gene and the GC content of itssequence was high, based on the data of the online software, we found ۲۹ sequences prone to Gquadruplexformation, among which ۱۴ regions had a score above ۲۰, which indicates that theprobability of the structure formation in these regions is very high. According to the recent evidence forthe in vivo location and role of DNA G-quadruplexes in several cellular pathways including DNAreplication and gene expression, effective treatment strategies can be defined for drug design andtargeted treatment of the diseases. Inhibiting this gene via G-quadruplex structures providesopportunities for shutting down pathways associated with tumor development and metastasis.

کلیدواژه ها:

RING۱ ، Regulation of gene expression ، G-quadruplex structure ، In Silico analysis