Bioinformatics Analysis of flavanone-3՛-hydroxylase (F3՜H) in Silybum marianum

Milk thistle (Silybum marianum), a member of the Asteraceae family, is known for its use inthe treatment of liver diseases due to its main component, silymarin. The biosynthesis of silymarininvolves the enzyme flavanone-3՛-hydroxylase (F3'H), which is encoded by a gene consisting of 1557nucleotides with a 51.87% cytosine-guanine content. The mRNA of this gene comprises 1317 basepairs, encoding a protein of 349 amino acids in length. The promoter sequence of the F3'H gene contains19 predicted regulatory elements, with the TATA-box and CAAT-box being the most prominent.Additionally, the gene exhibits several light response regulatory elements. According to the ProtParamdatabase, the protein encoded by this gene has an aliphatic index of 99.32, a predicted isoelectric pointof 7.87, and a molecular weight of 48.320 KD. The GRAVY range was calculated to be -0.059.Furthermore, the protein contains 49 negatively charged residues (Asp and Glu) and 50 positivelycharged residues (Arg and Lys). Its extinction coefficient, measured in water at 280 nm, is 42190 M-1cm-1. The protein was found to have an instability index of 34.65, classifying it as stable.

کلیدواژه های Bioinformatics Analysis of flavanone-3՛-hydroxylase (F3՜H) in Silybum marianum:

Flavanone-3՛-hydroxylase ، Protein ، Medicinal plants ، Secondary metabolite ، Silybum marianum