Bioinformatics Analysis of flavanone-۳՛-hydroxylase (F۳՜H) in Silybum marianum

Milk thistle (Silybum marianum), a member of the Asteraceae family, is known for its use inthe treatment of liver diseases due to its main component, silymarin. The biosynthesis of silymarininvolves the enzyme flavanone-۳՛-hydroxylase (F۳'H), which is encoded by a gene consisting of ۱۵۵۷nucleotides with a ۵۱.۸۷% cytosine-guanine content. The mRNA of this gene comprises ۱۳۱۷ basepairs, encoding a protein of ۳۴۹ amino acids in length. The promoter sequence of the F۳'H gene contains۱۹ predicted regulatory elements, with the TATA-box and CAAT-box being the most prominent.Additionally, the gene exhibits several light response regulatory elements. According to the ProtParamdatabase, the protein encoded by this gene has an aliphatic index of ۹۹.۳۲, a predicted isoelectric pointof ۷.۸۷, and a molecular weight of ۴۸.۳۲۰ KD. The GRAVY range was calculated to be -۰.۰۵۹.Furthermore, the protein contains ۴۹ negatively charged residues (Asp and Glu) and ۵۰ positivelycharged residues (Arg and Lys). Its extinction coefficient, measured in water at ۲۸۰ nm, is ۴۲۱۹۰ M-۱cm-۱. The protein was found to have an instability index of ۳۴.۶۵, classifying it as stable.

کلیدواژه ها:

Flavanone-۳՛-hydroxylase ، Protein ، Medicinal plants ، Secondary metabolite ، Silybum marianum